منابع مشابه
A knockout may not always be a knockout.
To the Editor: In a recent issue of Circulation, Hoorntje et al1 presented data on a family with a duplication in exon 4 of HERG. A consanguineous marriage resulted in 2 homozygous offspring: one died before birth and the other had a severe cardiac phenotype characterized by arrhythmias and conduction defects. The importance of these findings regarding the role of HERG in the heart is clear. Th...
متن کاملMouse knockout models of hypertension.
Gene-targeting technology allows the planned alteration of any gene in the mouse genome and has been very successfully employed to study the function of numerous gene products in a complete animal. The method includes the design of a suitable targeting construct, its transfection into pluripotential embryonic stem cells, selection for cells in which one allele of the endogenous gene has been ex...
متن کاملNew mouse is a knockout.
The potential of cis-diamminedichloroplatinum(II) (CDDP), trans-di(2-nitroimidazole)dichloro-platinum(II) (NIPt),
متن کاملA new SGK1 knockout mouse.
THE SERUM AND GLUCOCORTICOIDregulated Kinase (SGK1) is a component of the pathway mediating activation of the epithelial sodium channel, ENaC, in the aldosterone-sensitive distal nephron (ASDN). Aldosterone is released when the body needs more sodium or when blood pressure is low, leading to increased SGK1 transcription and translation (7, 23). SGK1 phosphorylates the E3 ubiquitin ligase Nedd4-...
متن کاملGeneration of global Spata19 knockout mouse using CRISPR/Cas9 nickase technology
Introduction: SPATA19 gene is expressed in developmental stages of testis and some organs, but so far its function has only been examined in the testis. In this study, we provided an effective pathway for the generation of these mice using new CRISPR / Cas9 nickase method while generating Spata19 knockout mice for future studies in other organs. Materials and Methods: CRISPR / Cas9 nickase plas...
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ژورنال
عنوان ژورنال: Nature
سال: 2011
ISSN: 0028-0836,1476-4687
DOI: 10.1038/474262a